000			01513nam a22001697a 4500
008			220929b |||||||| |||| 00| 0 eng d
020			_a9783319162584 - hbk
100			_aTill, Bradley J (et.al...)
245			_aLow-cost methods for molecular characterization of mutant plants : _b tissue desiccation, DNA extraction and mutation discovery: protocols _cTill, Bradley J; Till, Bradley J.,; Beshir, Mayada M.,; Hofinger, Bernhard J.,
260			_aNew York : _bSpringer, _c2015
300			_ax, 35p., illus., _bincludes references
520			_aThis book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits
650			_a1. Agriculture - General
650			_a2. Biochemical engineering
650			_a3. Biological Techniques
942			_cBK
999			_c8658 _d8658