Low-cost methods for molecular characterization of mutant plants : (Record no. 8658)

MARC details
000 -LEADER
fixed length control field 01513nam a22001697a 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 220929b |||||||| |||| 00| 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
ISBN 9783319162584 - hbk
100 ## - MAIN ENTRY--AUTHOR
Author Till, Bradley J (et.al...)
245 ## - TITLE STATEMENT
Title Low-cost methods for molecular characterization of mutant plants :
Remainder of title tissue desiccation, DNA extraction and mutation discovery: protocols
Statement of responsibility, etc Till, Bradley J; Till, Bradley J.,; Beshir, Mayada M.,; Hofinger, Bernhard J.,
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication New York :
Name of publisher Springer,
Date of publication 2015
300 ## - COLLATION
Pagination x, 35p., illus.,
Other physical details includes references
520 ## - SUMMARY, ETC.
Summary, etc This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits
650 ## - TRACINGS
Main Subject 1. Agriculture - General
650 ## - TRACINGS
Main Subject 2. Biochemical engineering
650 ## - TRACINGS
Main Subject 3. Biological Techniques
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Item type Books
Holdings
Source of classification or shelving scheme Not for loan Permanent location Current location Shelving location Date acquired Accession No. Full call number Barcode. Copy number Koha item type
Library of Congress Classification   Kwara State University Library Kwara State University Library Main Library 2022-09-29 018964 - 01 QK981.4 .L69 2015 018964 - 01 01 Books
Library of Congress Classification   Kwara State University Library Kwara State University Library Main Library 2022-09-29 018964 - 02 QK981.4 .L69 2015 018964 - 02 02 Books